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- Title
Allele resolution of HLA-A using oligonucleotide probes in a two-stage typing strategy.
- Authors
Williams, F; Meenagh, A; Maxwell, A P; Middleton, D
- Abstract
High-resolution polymerase chain reaction using sequence-specific oligonucleotide probes (PCR-SSOP) typing methods for HLA-A identification have been established. The four systems, which operate independently of each other, are intended for use as secondary typing systems following HLA-A identification with a medium-resolution PCR-SSOP technique. The systems, all using digoxigenin-labelled probes, are based on group specific amplifications for resolution of: i) HLA-A*29 & -A*33; ii) HLA-A*24 & -A*30; and iii) HLA-A*26, -A*25, -A*11, -A*34, -A*66 and -A*68 alleles, respectively. The fourth system, for the detection of HLA-A*02 alleles, is a modification of a previously reported PCR-SSOP subtyping system. The methods have been applied to individuals from the local bone marrow registry and HLA-A allele frequencies for the Northern Ireland population have been established.
- Publication
Tissue antigens, 1999, Vol 54, Issue 1, p59
- ISSN
0001-2815
- Publication type
Journal Article
- DOI
10.1034/j.1399-0039.1999.540107.x