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- Title
On-line monitoring of the methanol concentration in Pichia pastoris cultures producing an heterologous lipase by sequential injection analysis.
- Authors
Surribas, A; Cos, O; Montesinos, J L; Valero, F
- Abstract
An automated sequential injection analysis (SIA) system using stop-flow technique was developed to determine methanol concentration by means of the enzymatic reactions of alcohol oxidase and peroxidase. Its application as an on-line device for monitoring Pichia pastoris fermentations producing an heterologous protein was demonstrated. Linear response, observed up to 2 g l(-1), was reached by including a dilution chamber in the SIA manifold. The sampling frequency was 7 analyses per hour with a relative standard deviation lower than 4%.
- Publication
Biotechnology letters, 2003, Vol 25, Issue 21, p1795
- ISSN
0141-5492
- Publication type
Journal Article
- DOI
10.1023/a:1026228518418