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- Title
Paraquat resistance of transgenic tobacco plants over-expressing theOchrobactrum anthropipqrAgene.
- Authors
Jinki Jo; Sung-Hye Won; Daeyoung Son; Byung-Hyun Lee
- Abstract
Transgenic tobacco plants over-expressing theOchrobactrum anthropipqrAgene, which encodes a membrane transporter mediating resistance to paraquat, were generated. Transgenic plants displayed higher resistance against paraquat than wild-type plants, as estimated by plant viability, ion leakage and chlorophyll loss, but no resistance against other active oxygen generators, such as H2O2 and menadione. Moreover, lower levels of paraquat accumulated in transgenic plants, compared to wild-type plants, indicating that the PqrA protein detoxifies paraquat either via increased efflux or decreased uptake of the herbicide, but not by removing active oxygen species. The results collectively demonstrate that the bacterial paraquat resistance gene,pqrA, can be functionally expressed in plant cells, and utilized for the development of paraquat-resistant crop plants.
- Publication
Biotechnology Letters, 2004, Vol 26, Issue 18, p1391
- ISSN
0141-5492
- Publication type
Academic Journal
- DOI
10.1023/B:BILE.0000045638.82348.7a