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- Title
Apoptotic cell death kinetics in vitro depend on the cell types and the inducers used.
- Authors
Wolbers, F; Buijtenhuijs, P; Haanen, C; Vermes, I
- Abstract
In vitro exposure of cells to a fluorochrome-labeled inhibitor of caspases (FLICA) labels cells after caspase activation and arrests further progress of apoptotic cell death. The labeled apoptotic cells can be quantified in relation to time of apoptosis induction with flow cytometry. Loss of membrane integrity (late apoptosis and cell death) was measured with exposure to propidium iodide (PI). From the labeling patterns with FLICA and PI the apoptotic cell death kinetics was calculated.
- Publication
Apoptosis : an international journal on programmed cell death, 2004, Vol 9, Issue 3, p385
- ISSN
1360-8185
- Publication type
Journal Article
- DOI
10.1023/b:appt.0000025816.16399.7a