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- Title
Gene expression profiling of p53-sensitive and -resistant tumor cells using DNA microarray.
- Authors
Maxwell, S A; Davis, G E
- Abstract
Overexpression of wild-type p53 in ECV-304 tumor cells induced extensive apoptosis and the eventual death of nearly all of the cells. We generated ECV-304 cells resistant to p53-induced apoptosis as a strategy to identify novel genes that might be relevant to p53-mediated apoptosis. ECV-304 cells resistant to p53 were isolated by repeated infections with a recombinant p53 adenovirus and were designated as DECV. The expression of 5,730 genes in p53-resistant (DECV) and p53-sensitive ECV-304 cells were profiled by DNA microarray analysis. We report here the expression of 80 genes that differed by 2-fold or more between sensitive and resistant cells upregulated for p53. Many of these differentially expressed genes are regulated by p53 in ECV-304 and H1299 p53-null cells. Our analysis identifies many new potential targets for p53 that play roles in cell cycle regulation, DNA repair, redox control, cell adhesion, apoptosis, and differentiation.
- Publication
Apoptosis : an international journal on programmed cell death, 2004, Vol 9, Issue 2, p171
- ISSN
1360-8185
- Publication type
Journal Article
- DOI
10.1023/B:APPT.0000018799.20120.38