Title

Large-scale production and purification of recombinant Galanthus nivalis agglutinin (GNA) expressed in the methylotrophic yeast Pichia pastoris.

Authors

Baumgartner, Philippe; Harper, Karen; Raemaekers, Romaan J.M.; Durieux, Alain; Gatehouse, Angharad M.R.; Davies, Howard V.; Taylor, Mark A.

Abstract

The gene coding for agglutinin from Galanthus nivalis (GNA) was expressed in, and secreted by, the methylotrophic yeast, Pichia pastoris. Transformants of P. pastoris were selected and a process to produce and purify gram quantities of recombinant GNA was developed. GNA was secreted at approximately 80 mg l-1 at the 200 l scale and was purified to 95% homogeneity using hydrophobic interaction chromatography. The recombinant protein was similar to the protein synthesised in plant with respect to structure and biological activity.

Subjects

COMMON snowdrop; AGGLUTININS; YEAST; GENES; RECOMBINANT proteins; PROTEIN synthesis

Publication

Biotechnology Letters, 2003, Vol 25, Issue 15, p1281

ISSN

0141-5492

Publication type

Academic Journal

DOI

10.1023/A:1025007901322