We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
RNA as a Source of Transposase for Sleeping Beauty-Mediated Gene Insertion and Expression in Somatic Cells and Tissues.
- Authors
Wilber, Andrew; Frandsen, Joel L.; Geurts, Jennifer L.; Largaespada, David A.; Hackett, Perry B.; McIvor, R. Scott
- Abstract
Sleeping Beauty (SB) is a DNA transposon capable of mediating gene insertion and long-term expression in vertebrate cells when co-delivered with a source of transposase. In all previous reports of SB-mediated gene insertion in somatic cells, the transposase component has been provided by expression of a co-delivered DNA molecule that has the potential for integration into the host cell genome. Integration and continued expression of a gene encoding SB transposase could be problematic if it led to transposon re-mobilization and reintegration. We addressed this potential problem by supplying the transposase-encoding molecule in the form of mRNA. We show that transposase-encoding mRNA can effectively mediate transposition in vitro in HT1080 cells and in vivo in mouse liver following co-delivery with a recoverable transposon or with a luciferase transposon. We conclude that in vitro-transcribed mRNA can be used as an effective source of transposase for SB-mediated transposition in mammalian cells and tissues.Molecular Therapy (2006) 13, 625–630; doi: 10.1016/j.ymthe.2005.10.014
- Publication
Molecular Therapy, 2006, Vol 13, Issue 3, p625
- ISSN
1525-0016
- Publication type
Academic Journal
- DOI
10.1016/j.ymthe.2005.10.014