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- Title
Insulinomas and expression of an insulin splice variant.
- Authors
Minn, Alexandra H; Kayton, Mark; Lorang, Dominique; Hoffmann, Steven C; Harlan, David M; Libutti, Steven K; Shalev, Anath
- Abstract
Insulinomas are beta-cell tumours characterised by uncontrolled insulin secretion even in the presence of hypoglycaemia. However, the mechanisms allowing such excessive insulin secretion are not known. Insulin secretion can occur only when the beta-cell insulin stores have been replenished by insulin biosynthesis, which is mainly controlled by translation. Such specific translational regulation often involves the 5' untranslated region. We have identified an insulin splice variant in isolated human pancreatic islets of non-diabetic donors that retains 26 bp of intron 1 and thereby changes the 5' untranslated region, but leaves the coding region unchanged. This splice variant has increased translation efficiency in vitro and in vivo compared with native insulin mRNA. However, splice variant expression is less than 1% of native insulin mRNA in normal islets.
- Publication
Lancet (London, England), 2004, Vol 363, Issue 9406, p363
- ISSN
1474-547X
- Publication type
Journal Article
- DOI
10.1016/S0140-6736(04)15438-X