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Title

Annotation and De Novo Sequence Characterization of Extracellular β-Fructofuranosidase from <italic>Penicillium chrysogenum</italic> Strain HKF42.

Authors

Gujar, Vaibhav V.; Fuke, Priya; Khardenavis, Anshuman A.; Purohit, Hemant J.

Abstract

The genome of a fungal strain &lt;italic&gt;Penicillium chrysogenum&lt;/italic&gt; strain HKF42, which can grow on 20% sucrose has been annotated for 7595 protein coding sequences. On mining of CAZymes, we could annotate a β-fructofuranosidase gene responsible for fructo-oligosaccharides (FOS) synthesis which is a known prebiotic. The enzyme activity was demonstrated and validated with the generation of FOS as kestose and nystose.

Subjects

PENICILLIUM chrysogenum; INVERTASE genetics; FUNGAL genomes; FRUCTOOLIGOSACCHARIDES; PREBIOTICS

Publication

Indian Journal of Microbiology, 2018, Vol 58, Issue 2, p227

ISSN

0046-8991

Publication type

Academic Journal

DOI

10.1007/s12088-017-0704-y

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