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- Title
A mini-IRES sequence for stringent selection of high producers.
- Authors
Yan, Jun; Yang, Hailin; Yue, Guohua; Gao, Wenda
- Abstract
Internal Ribosome Entry Site (IRES) sequences have been widely used to link the expression of two independent proteins on the same mRNA transcript. Genes encoding fluorescent proteins or drug-resistance enzymes are usually placed downstream of IRES, serving as expression indicators or selection markers. In biological applications where the upstream gene-of-interest is to be expressed at extremely high levels, it is often desirable to purposely reduce IRES downstream gene expression to economize the cellular resources and/or to generate more stringent selection pressure. Here we describe a miniature IRES mutant sequence (IRESmut3) with dramatically diminished co-translational efficiency to fulfill these purposes.
- Publication
Journal of biosciences, 2013, Vol 38, Issue 2, p245
- ISSN
0973-7138
- Publication type
Journal Article
- DOI
10.1007/s12038-013-9323-x