Construction of Synthetic Promoter-Based Expression Cassettes for the Production of Cadaverine in Recombinant Corynebacterium glutamicum.

Oh, Young; Choi, Jae; Kim, Eun; Song, Bong; Jeong, Ki; Park, Kyungmoon; Kim, Il-Kwon; Woo, Han; Lee, Seung; Park, Si

Corynebacterium glutamicum is an important microorganism in the biochemical industry for the production of various platform chemicals. However, despite its importance, a limited number of studies have been conducted on how to constitute gene expression cassettes in engineered C. glutamicum to obtain desired amounts of the target products. Therefore, in this study, six expression cassettes for the expression of the second lysine decarboxylase of Escherichia coli, LdcC, were constructed using six synthetic promoters with different strengths and were examined in C. glutamicum for the production of cadaverine. Among six expression cassettes, the expression of the E. coli ldcC gene under the P promoter supported the highest production of cadaverine in flask and fed-batch cultivations. A fed-batch fermentation of recombinant C. glutamicum expressing E. coli ldcC gene under the P promoter resulted in the production of 40.91 g/L of cadaverine in 64 h. This report is expected to contribute toward developing engineered C. glutamicum strains to have desired features.

GENE expression; PROMOTERS (Genetics); CORYNEBACTERIUM glutamicum; LYSINE decarboxylase; ESCHERICHIA coli

Applied Biochemistry & Biotechnology, 2015, Vol 176, Issue 7, p2065

0273-2289

Academic Journal

10.1007/s12010-015-1701-4