Title

Role of a Repeated Hexapeptide Motif GIHFAP Near C-terminus in Assembly, Stability, and Activity of 'HCH Dehydrochlorinase LinA'.

Authors

Macwan, Ankit; Srivastava, Nidhi; Javed, Saleem; Kumar, Ashwani

Abstract

Enzyme 'hexachlorocyclohexane (HCH) dehydrochlorinase LinA' mediates first step of aerobic microbial degradation of a chlorinated insecticide γ-HCH. The archetypal LinA-type1 consists of 156 amino acids that include a directly repeated hexapeptide motif GIHFAP at positions 141-146 and 148-153. Analysis of a series of LinA mutants, containing none, one, two, or three units of this repeated motif revealed that two units, as present in wild-type LinA, are required for its optimal activity and stability. Moreover, the presence of a bend in its secondary structure due to a proline residue that precedes the distal repeated unit contributes to enhanced LinA activity.

Subjects

HEXACHLOROCYCLOHEXANES; INSECTICIDES; AMINO acids; PESTICIDES; HEXAPEPTIDES; ENZYMES

Publication

Applied Biochemistry & Biotechnology, 2013, Vol 169, Issue 4, p1397

ISSN

0273-2289

Publication type

Academic Journal

DOI

10.1007/s12010-012-0035-8