Title

Heterologous Expression and Characterization of an Endoglucanase from a Symbiotic Protist of the Lower Termite, Reticulitermes speratus.

Authors

Todaka, Nemuri; Lopez, Crisanto M.; Inoue, Tetsushi; Saita, Kanako; Maruyama, Jun-ichi; Arioka, Manabu; Kitamoto, Katsuhiko; Kudo, Toshiaki; Moriya, Shigeharu

Abstract

RsSymEG, an endoglucanase of glycosyl hydrolase family (GHF) 7 encoded by a transcript isolated from the symbiotic protist of the termite Reticulitermes speratus, is expressed in Aspergillus oryzae. Interestingly, purified RsSymEG1 has a relatively higher specific activity (603 µmol min-1 mg-1 protein) and Vmax value (769.6 unit/mg protein) than previously reported data for GHF7 endoglucanase of Trichoderma ressei. It also has the same Km value (1.97 mg/ml) with Clostridium cellulolyticum enzymes that contain cellulose binding module, a property indicative of high affinity to substrate, though no cellulose binding module is found within it. Thin-layer chromatography analysis revealed that RsSymEG1 preferentially hydrolyzes the β-1,4-cellulosic linkage of cellodextrins into cellobiose and glucose.

Subjects

GLUCANS; PROTISTA; ASPERGILLUS; CLOSTRIDIUM; THIN layer chromatography; HYDROLYSIS; RETICULITERMES

Publication

Applied Biochemistry & Biotechnology, 2010, Vol 160, Issue 4, p1168

ISSN

0273-2289

Publication type

Academic Journal

DOI

10.1007/s12010-009-8626-8