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- Title
Effects of vitamin C on characteristics retaining of in vitro-cultured mouse adipose-derived stem cells.
- Authors
Wei, Chao; Liu, Xing; Tao, Jia; Wu, Ronghua; Zhang, Pengfei; Bian, Yani; Li, Yunsheng; Fang, Fugui; Zhang, Yunhai
- Abstract
Adipose-derived stem cells (ADSCs), a subset of mesenchymal stem cells, have promising potential for regenerative medicine applications. However, the efficient culture of mouse adipose-derived stem cells (mADSCs) is complicated or impracticable and many properties of mADSCs are still unknown. Here, we report that vitamin C (Vc) is available for the long-term culture of mADSCs in vitro. Compared with that cultured without Vc, mADSCs growing in Vc-added media proliferate faster. The occurrence of replicative senescence and transformation of Vc-treated mADSCs is also postponed. Vc also enhanced the secretory activity of collagen and adipogenic differentiation ability of mADSCs. Moreover, the expression of CD44, Sca-1, and CD105 is higher in Vc-treated mADSCs than nontreated ones. We also found that genes related to proliferation, secretion, and pluripotency are all promoted in Vc-treated mADSCs. However, the adipogenesis ability and expression of CD44, Sca-1, and CD105 decreased when passage increased from very low passages, in parallel to the downregulation of closed-related gene expression. Our results indicate that Vc is essential for the maintenance of original properties of mADSCs in vitro; additional insights into the function of Vc on mADSCs are provided. Furthermore, as the passage increased in six passages, the characteristics of mADSCs with Vc addition were also revealed.
- Publication
In vitro cellular & developmental biology. Animal, 2014, Vol 50, Issue 1, p75
- ISSN
1543-706X
- Publication type
Journal Article
- DOI
10.1007/s11626-013-9673-6