We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Lhx8 promote differentiation of hippocampal neural stem/progenitor cells into cholinergic neurons in vitro.
- Authors
Shi, Jinhong; Li, Haoming; Jin, Guohua; Zhu, Peipei; Tian, Meiling; Qin, Jianbing; Tan, Xuefeng; Zhao, Shuqing; Wang, Fuyu; Hua, Yurong; Xiao, Yong
- Abstract
Lhx8, also named L3, is a recently identified member of the LIM homeobox gene family. Previously, we found acetylcholinesterase (AChE)-positive cells in fimbria-fornix (FF) transected rat hippocampal subgranular zone (SGZ). In the present study, we detected choline acetyltransferase (ChAT)-positive cholinergic cells in hippocampal SGZ after FF transaction, and these ChAT-positive cells were double labeled by Lhx8. Then we overexpressed Lhx8 during neural differentiation of hippocampal neural stem/progenitor cells on adherent conditions using lentivirus Lenti6.3-Lhx8. The result indicated that overexpression of Lhx8 did not affect the proportion of MAP2-positive neurons, but increased the proportion of ChAT-positive cells in vitro. These results suggested that FF-transected hippocampal niche promoted the ChAT/Lhx8-positive cholinergic neurons generation in rodent hippocampus, and Lhx8 was not associated with the MAP2-positive neurons differentiation on adherent conditions, but played a role in the specification of cholinergic neurons derived from hippocampal neural stem/progenitor cells in vitro.
- Publication
In vitro cellular & developmental biology. Animal, 2012, Vol 48, Issue 10, p603
- ISSN
1543-706X
- Publication type
Journal Article
- DOI
10.1007/s11626-012-9562-4