Combined treatment with parathyroid hormone (1-34) and beta-tricalcium phosphate had an additive effect on local bone formation in a rat defect model.

Tao, Zhou-Shan; Tu, Kai-kai; Huang, Zheng-Liang; Zhou, Qiang; Sun, Tao; Xu, Hong-Ming; Zhou, Yu-Long; Lv, Yang-Xun; Cui, Wei; Yang, Lei

The objective of this study was to evaluate the effect of following combined treatment with parathyroid hormone (1-34) (PTH) and beta-tricalcium phosphate (β-TCP) on local bone formation in a rat 3-mm critical-size defect at distal femur. Twelve weeks after bilateral ovariectomy (OVX) and sham operation (sham), all animals were randomly divided into four groups: group OVX, group OVX   β-TCP, group sham, and group sham   β-TCP, then all rats underwent bone defect in the bilateral distal femurs, and β-TCP were implanted into critical-sized defects for group OVX   β-TCP and group sham   β-TCP. After defect operation, all animals were received following subcutaneous injections with PTH (60 μg/kg, three times a week) until death at 4 and 8 weeks. The defected area in distal femurs of rats was harvested for evaluation by histology, micro-CT, and biomechanics. The results of our study show that systemic usage of PTH or local usage of β-TCP can increase the healing of defects in OVX or sham rats. Furthermore, treatments with PTH and β-TCP showed a stronger effect on accelerating the local bone formation than used alone. Osteoporosis can limit the function of PTH and/or β-TCP. The results from our study demonstrate that combination of PTH and β-TCP had an additive effect on local bone formation in non-osteoporosis and/or osteoporosis rats.

PARATHYROID hormone; CALCIUM phosphate; FEMUR; OVARIECTOMY; BONE diseases; SUBCUTANEOUS infusions; HISTOLOGY

Medical & Biological Engineering & Computing, 2016, Vol 54, Issue 9, p1353

0140-0118

Academic Journal

10.1007/s11517-015-1402-8