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- Title
Cotton plants expressing CYP6AE14 double-stranded RNA show enhanced resistance to bollworms.
- Authors
Ying-Bo Mao; Xiao-Yuan Tao; Xue-Yi Xue; Ling-Jian Wang; Xiao-Ya Chen
- Abstract
RNA interference (RNAi) plays an important role in regulating gene expression in eukaryotes. Previously, we generated Arabidopsis and tobacco plants expressing double-stranded RNA (dsRNA) targeting a cotton bollworm ( Helicoverpa armigera) P450 gene, CYP6AE14. Bollworms fed on transgenic dsCYP6AE14 plants showed suppressed CYP6AE14 expression and reduced growth on gossypol-containing diet (Mao et al., in Nat Biotechnol 25: 1307-1313, ). Here we report generation and analysis of dsRNA-expressing cotton ( Gossypium hirsutum) plants. Bollworm larvae reared on T2 plants of the ds6- 3 line exhibited drastically retarded growth, and the transgenic plants were less damaged by bollworms than the control. Quantitative reverse-transcription polymerase chain reaction (RT-PCR) showed that the CYP6AE14 expression level was reduced in the larvae as early as 4 h after feeding on the transgenic plants; accordingly, the CYP6AE14 protein level dropped. These results demonstrated that transgenic cotton plants expressing dsCYP6AE14 acquired enhanced resistance to cotton bollworms, and that RNAi technology can be used for engineering insect-proof cotton cultivar.
- Publication
Transgenic Research, 2011, Vol 20, Issue 3, p665
- ISSN
0962-8819
- Publication type
Academic Journal
- DOI
10.1007/s11248-010-9450-1