Title

Light-regulated betacyanin accumulation in euhalophyte Suaeda salsa calli.

Authors

Shu-Zhen Zhao; Hong-Zhao Sun; Min Chen; Bao-Shan Wang

Abstract

Suaeda salsa calli cultured in darkness for 28 days were transferred to Murashige and Skoog (MS) media containing 0.5 mg l−1 Thidiazuron (TDZ) and 1.0 mg l−1 naphthaleneacetic acid (NAA) under different light conditions for 10 days to investigate effect of light on betacyanin accumulation and its relation to activity and expression of tyrosinase and expression of dopa-4,5-dioxygenase gene ( DODA). Both light quality and quantity affected betacyanin synthesis of S. salsa calli. 80 μmol m−2 s−1 of white light intensity was optimal for cell growth and betacyanin accumulation of S. salsa calli. DODA mRNA levels and tyrosinase activity were consistent with the response of betacyanin content to different light quality. However, the protein levels of tyrosinase extracted from S. salsa calli response to different light quality were constant. These results suggest that betacyanin metabolism in S. salsa calli is regulated by different light quality through the regulation of genes such as DODA and tyrosinase activity but not via alteration of the protein amount of tyrosinase.

Subjects

PLANT pigments; MESSENGER RNA; COLOR of plants; NAPHTHALENEACETIC acid; PHENOL oxidase; PLANT extracts

Publication

Plant Cell, Tissue & Organ Culture, 2010, Vol 102, Issue 1, p99

ISSN

0167-6857

Publication type

Academic Journal

DOI

10.1007/s11240-010-9710-z