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- Title
ARABIDILLO proteins have a novel and conserved domain structure important for the regulation of their stability.
- Authors
Nibau, Cândida; Gibbs, Daniel J; Bunting, Karen A; Moody, Laura A; Smiles, Emma J; Tubby, Jennifer A; Bradshaw, Susan J; Coates, Juliet C
- Abstract
ARABIDILLO proteins are F-box-Armadillo (ARM) proteins that regulate root branching in Arabidopsis. Many F-box proteins in plants, yeast and mammals are unstable. In plants, the mechanism for this instability has not been fully investigated. Here, we show that a conserved family of plant ARABIDILLO-related proteins has a unique domain structure consisting of an F-box and leucine-rich repeats (LRRs) followed by ARM-repeats. The LRRs are similar to those found in other plant and animal F-box proteins, including cell cycle proteins and hormone receptors. We demonstrate that the LRRs are required for ARABIDILLO1 function in vivo. ARABIDILLO1 protein is unstable: we show that ARABIDILLO1 protein is associated with ubiquitin and is turned over by the proteasome. Both the F-box and LRR regions of ARABIDILLO1 appear to enable this turnover to occur. Application of known lateral root-regulating signals has no effect on ARABIDILLO1 stability. In addition, plants that lack or overexpress ARABIDILLO proteins respond normally to known lateral root-regulating signals. Thus, we suggest that the signal(s) regulating ARABIDILLO stability in vivo may be either highly specific or novel. The structural conservation between ARABIDILLOs and other plant and animal F-box proteins suggests that the stability of other F-box proteins may be controlled by similar mechanisms.
- Publication
Plant molecular biology, 2011, Vol 75, Issue 1-2, p77
- ISSN
1573-5028
- Publication type
Journal Article
- DOI
10.1007/s11103-010-9709-1