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- Title
Lyophilization of Protein-Loaded Polyelectrolyte Microcapsules.
- Authors
Temmerman, Marie-Luce; Rejman, Joanna; Grooten, Johan; Beer, Thomas; Vervaet, Chris; Demeester, Jo; Smedt, Stefaan
- Abstract
Purpose: To evaluate if lyophilization can be used to obtain a dry formulation of polyelectrolyte microcapsules, which have emerged as a new class of microparticles for the encapsulation and delivery of biomacromolecules. Methods: Microcapsules composed of dextran sulfate and poly-L-arginine were obtained by coating CaCO microparticles by means of the layer-by-layer technique. Microcapsules were lyophilized using different stabilizers; intactness was checked by CLSM and SEM. Horseradish peroxidase was encapsulated as model enzyme and retained activity after freeze-drying was determined using a fluorescence assay. Ovalbumin was encapsulated as model antigen; immunogenicity after lyophilization was evaluated in vitro by a T-cell proliferation assay and in vivo by measuring the antibody titer in mice. Results: The results clearly demonstrate the necessity of using polyols in the formulation to prevent rupture of the microcapsules and to preserve the activity of encapsulated enzymes. Lyophilized microcapsules appeared as a promising adjuvant for antigen delivery, as both in vitro as in vivo assays showed higher immune activation compared to free antigen. Conclusions: Lyophilization is a promising strategy towards improved stability of protein-loaded microcapsules.
- Publication
Pharmaceutical Research, 2011, Vol 28, Issue 7, p1765
- ISSN
0724-8741
- Publication type
Academic Journal
- DOI
10.1007/s11095-011-0411-z