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- Title
Characterization of a diffuse intrinsic pontine glioma cell line: implications for future investigations and treatment.
- Authors
Hashizume, Rintaro; Smirnov, Ivan; Liu, Sharon; Phillips, Joanna J; Hyer, Jeanette; McKnight, Tracy R; Wendland, Michael; Prados, Michael; Banerjee, Anu; Nicolaides, Theodore; Mueller, Sabine; James, Charles D; Gupta, Nalin
- Abstract
Diffuse intrinsic pontine gliomas arise almost exclusively in children, and despite advances in treatment, the majority of patients die within 2 years after initial diagnosis. Because of their infiltrative nature and anatomic location in an eloquent area of the brain, most pontine gliomas are treated without a surgical biopsy. The corresponding lack of tissue samples has resulted in a limited understanding of the underlying genetic and molecular biologic abnormalities associated with pontine gliomas, and is a substantial obstacle for the preclinical testing of targeted therapeutic agents for these tumors. We have established a human glioma cell line that originated from surgical biopsy performed on a patient with a pontine glioma. To insure sustainable in vitro propagation, tumor cells were modified with hTERT (human telomerase ribonucleoprotein reverse transcriptase), and with a luciferase reporter to enable non-invasive bioluminescence imaging. The hTERT modified cells are tumorigenic in athymic rodents, and produce brainstem tumors that recapitulate the infiltrative growth of brainstem gliomas in patients.
- Publication
Journal of neuro-oncology, 2012, Vol 110, Issue 3, p305
- ISSN
1573-7373
- Publication type
Journal Article
- DOI
10.1007/s11060-012-0973-6