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- Title
Overexpression of PGC-1β improves insulin sensitivity and mitochondrial function in 3T3-L1 adipocytes.
- Authors
Chun-Lin Gao; Guang-Ling Liu; Shi Liu; Xiao-Hui Chen; Chen-Bo Ji; Chun-Mei Zhang; Zheng-Kun Xia; Xi-rong Guo
- Abstract
The co-transcription factor peroxisome proliferator-activated receptor γ coactivator-1β (PGC-1β) was first identified in 2002. Although the function of PGC-1β in white adipose tissue (WAT) is largely unknown, it has been studied extensively in the liver, cardiac muscle, and skeletal muscle. Herein, we investigated PGC-1β overexpression in 3T3-L1 adipocytes. The main findings were as follows: (i) 3T3-L1 adipocytes overexpressing PGC-1β showed improved insulin sensitivity and elevated insulin-stimulated glucose uptake; (ii) mitochondrial cristae became broader and more ordered, additional smaller mitochondria emerged, mitochondrial DNA increased, and fission 1 protein (Fis1) mRNA expression was greatly elevated; (iii) intracellular ATP levels increased, but no changes were observed in mitochondrial membrane potential, uncoupling protein (UCP) mRNA expression, or reactive oxygen species (ROS) production; and (iv) mitochondrial metabolism factors, namely, acetyl-coenzyme A carboxylase 2 (ACC2) and hexokinase 2 (HK2) were downregulated, while cytochrome c oxidase subunit IV (COX IV) was upregulated. In conclusion, PGC-1β affects not only insulin sensitivity but also mitochondrial biogenesis and function. We believe that the role of PGC-1β is distinct from that of PGC-1α in WAT.
- Publication
Molecular & Cellular Biochemistry, 2011, Vol 353, Issue 1/2, p215
- ISSN
0300-8177
- Publication type
Academic Journal
- DOI
10.1007/s11010-011-0789-2