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- Title
Analyzing diurnal and age-related pheromone emission of the olive fruit fly, Bactrocera oleae by sequential SPME-GCMS analysis.
- Authors
Levi-Zada, Anat; Nestel, David; Fefer, Daniela; Nemni-Lavy, Esther; Deloya-Kahane, Inbal; David, Maayan
- Abstract
The olive fruit fly, Bactrocera oleae (Diptera: Tephritidae), uses 1,7-dioxaspiro[5.5]undecane ("olean"), produced primarily by females, as a sex pheromone. We used sequential solid phase microextraction-gas chromatography mass spectrometry (SPME-GCMS) analysis to show that female olive flies release about 1000 ng of pheromone at the onset of scotophase for several weeks, while males release about 1/100 as much during the first week after eclosion. The present research demonstrates details of employing SPME-GCMS with the partially known pheromone system of the olive fruit fly as a model for pheromone identification and diurnal release patterns in insects, especially fruit flies. The sequential SPME-GCMS method will readily allow detection and semi-quantification of semiochemicals released by insects in minute amounts throughout the diurnal cycle.
- Publication
Journal of chemical ecology, 2012, Vol 38, Issue 8, p1036
- ISSN
1573-1561
- Publication type
Journal Article
- DOI
10.1007/s10886-012-0167-x