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- Title
Functional centromeres in soybean include two distinct tandem repeats and a retrotransposon.
- Authors
Tek, Ahmet L; Kashihara, Kazunari; Murata, Minoru; Nagaki, Kiyotaka
- Abstract
The centromere as a kinetochore assembly site is fundamental to the partitioning of genetic material during cell division. In order to determine the functional centromeres of soybean, we characterized the soybean centromere-specific histone H3 (GmCENH3) protein and developed an antibody against the N-terminal end. Using this antibody, we cloned centromere-associated DNA sequences by chromatin immunoprecipitation. Our analyses indicate that soybean centromeres are composed of two distinct satellite repeats (GmCent-1 and GmCent-4) and retrotransposon-related sequences (GmCR). The possible allopolyploid origin of the soybean genome is discussed in view of the centromeric satellite sequences present.
- Publication
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 2010, Vol 18, Issue 3, p337
- ISSN
1573-6849
- Publication type
Journal Article
- DOI
10.1007/s10577-010-9119-x