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- Title
DA-9601 inhibits activation of the human mast cell line HMC-1 through inhibition of NF-κB.
- Authors
S. Lee; H.-H. Park; H.-Y. Son; J.-H. Ha; M.-G. Lee; T.-Y. Oh; D. Sohn; T. Jeong; J.-K. Son; C.-D Jun
- Abstract
Abstract??Mast cell-mediated allergic inflammation is involved in many diseases such as asthma, sinusitis, and rheumatoid arthritis. Mast cells induce synthesis and production of pro-inflammatory cytokines including tumor necrosis factor (TNF)-?, interleukin (IL)-1? and IL-6 with immune regulatory properties. The formulated ethanol extract ofArtemisia asiaticaNakai (DA-9601) has been reported to have antioxidative and anti-inflammatory activities. In this report, we investigated the effect of DA-9601 on the expression of pro-inflammatory cytokines by the activated human mast cell line HMC-1 and studied its possible mechanisms of action. DA-9601 dose-dependently decreased the gene expression and production of TNF-?, IL-1?, and IL-6 on phorbol 12-myristate 13-acetate (PMA)- and calcium ionophore A23187-stimulated HMC-1 cells. In addition, DA-9601 attenuated PMA- and A23187-induced activation of NF-?B as indicated by inhibition of degradation of I?B?, nuclear translocation of NF-?B, NF-?B/DNA binding, and NF-?B-dependent gene reporter assay. Ourin vitrostudies provide evidence that DA-9601 might contribute to the treatment of mast cell-derived allergic inflammatory diseases.
- Publication
Cell Biology & Toxicology, 2007, Vol 23, Issue 2, p105
- ISSN
0742-2091
- Publication type
Academic Journal
- DOI
10.1007/s10565-006-0103-3