Title

Somatic embryogenesis in Araucaria angustifolia.

Authors

A. Wendt dos Santos; N. Steiner; M. Guerra; K. Zoglauer; B. Moerschbacher

Abstract

Abstract  Immature and mature zygotic embryos were used as source of explants for induction of somatic embryogenesis in Araucaria angustifolia. Embryogenic cultures (EC) were only obtained from immature zygotic embryos. Basic medium, carbon source, and genotype showed a significant influence on the formation of stage I somatic embryos (SE). When EC were submitted to maturation conditions, SE continued their individual development until stage II, but mature embryos were not obtained. Proteins secreted by embryogenic cultures were, to a certain degree, genotype specific and included an extracellular class IV chitinase and β-1-3-glucanase.

Subjects

ARAUCARIACEAE; PLANT tissue culture; BRAZILIAN pine; SOMATIC embryogenesis

Publication

Biologia Plantarum, 2008, Vol 52, Issue 1, p195

ISSN

0006-3134

Publication type

Academic Journal

DOI

10.1007/s10535-008-0044-1