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- Title
A genome walking strategy for the identification of nucleotide sequences adjacent to known regions.
- Authors
Wang, Hailong; Yao, Ting; Cai, Mei; Xiao, Xiuqing; Ding, Xuezhi; Xia, Liqiu
- Abstract
To identify the transposon insertion sites in a soil actinomycete, Saccharopolyspora spinosa, a genome walking approach, termed SPTA-PCR, was developed. In SPTA-PCR, a simple procedure consisting of TA cloning and a high stringency PCR, following the single primer-mediated, randomly-primed PCR, can eliminate non-target DNA fragments and obtain target fragments specifically. Using SPTA-PCR, the DNA sequence adjacent to the highly conserved region of lectin coding gene in onion plant, Allium chinense, was also cloned.
- Publication
Biotechnology letters, 2013, Vol 35, Issue 2, p279
- ISSN
1573-6776
- Publication type
Journal Article
- DOI
10.1007/s10529-012-1076-3