We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Acetate accumulation through alternative metabolic pathways in ackA pta poxB triple mutant in E. coli B (BL21).
- Authors
Je-Nie Phue; Sang Jun Lee; Kaufman, Jeanne B.; Negrete, Alejandro; Shiloach, Joseph
- Abstract
Individual deletions of acs and aceA genes in E. coli B (BL21) showed little difference in the metabolite accumulation patterns but deletion of the ackA gene alone or together with pta showed acetic acid gradually accumulated to 3.1 and 1.7 g/l, respectively, with a minimal extended lag in bacterial growth and a higher pyruvate formation. Single poxB deletion in E. coli B (BL21) or additional poxB deletion in the ackA- pta mutants did not change the acetate accumulation pattern. When the acetate production genes ( ackA- pta- poxB) were deleted in E. coli B (BL21) acetate still accumulated. This may be an indication that perhaps acetate is not only a by-product of carbon metabolism; it is possible that acetate plays also a role in other cellular metabolite pathways. It is likely that there are alternative acetate production pathways.
- Publication
Biotechnology Letters, 2010, Vol 32, Issue 12, p1897
- ISSN
0141-5492
- Publication type
Academic Journal
- DOI
10.1007/s10529-010-0369-7