We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Cloning and characterization of CalS7 from Micromonospora echinospora sp. calichensis as a glucose-1-phosphate nucleotidyltransferase.
- Authors
Simkhada, Dinesh; Oh, Tae-Jin; Kim, Eui Min; Yoo, Jin Cheol; Sohng, Jae Kyung
- Abstract
The deoxysugar biosynthetic gene cluster of calicheamicin contains the calS7, which encodes glucose-1-phosphate nucleotidyltransferase and converts glucose-1-phosphate and nucleotides (NTP) to NDP-glucose and pyrophosphate. calS7 was expressed in Escherichia coli BL21(DE3), and the purified protein had significant thymidylyltransferase and uridylyltransferase activities as well, with some guanidylyltransferase activity but negligible cytidyl and adenyltransferase activity. The functions of thymidylyltransferase and uridylyltransferase were also verified using one-pot enzymatic synthesis of TMK and ACK. The products were analyzed by HPLC and ESI/MS, which showed peaks at m/z = 563 and 565 for TDP-D: -glucose and UDP-D-glucose, respectively, in negative mode.
- Publication
Biotechnology letters, 2009, Vol 31, Issue 1, p147
- ISSN
1573-6776
- Publication type
Journal Article
- DOI
10.1007/s10529-008-9844-9