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- Title
pGFPGUSPlus, a new binary vector for gene expression studies and optimising transformation systems in plants.
- Authors
Vickers, Claudia E; Schenk, Peer M; Li, Dongxue; Mullineaux, Philip M; Gresshoff, Peter M
- Abstract
A binary vector containing two reporter gene cassettes has been developed. This vector is ideal for optimising new plant transformation systems. Following optimisation, one of the reporter genes can be replaced with a gene of interest; the second can be used as a marker to confirm transgenic lines, and to estimate locus number and determine zygosity. This allows simple, efficient and economical screening for homozygous single-insert lines and azygous controls.
- Publication
Biotechnology letters, 2007, Vol 29, Issue 11, p1793
- ISSN
0141-5492
- Publication type
Journal Article
- DOI
10.1007/s10529-007-9467-6