We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Phytase expression in transgenic soybeans: stable transformation with a vector-less construct.
- Authors
Xiao Rong Gao; Guo Kun Wang; Qiao Su; Yan Wang; Li Jia An
- Abstract
A minimal linear gene cassette (35S-phytase gene-nos) with T-DNA borders was acquired by PCR and directly introduced into soybean through the pollen tube pathway. A total of 13% of T1 plants were positive for phyA by specific PCR. Southern blot analyses showed that phyA insertions were harbored stably in T2 progeny. Phytase expression level increased 2.5-fold over a 6-week period; its highest activity was 150 U/mg protein, compared to 56 U/mg protein in untransformed controls. Activity of phytase increased to 125 FTU/kg in T3 transgenic seeds as compared to 64 FTU/kg in wild-type plants.
- Publication
Biotechnology Letters, 2007, Vol 29, Issue 11, p1781
- ISSN
0141-5492
- Publication type
Academic Journal
- DOI
10.1007/s10529-007-9439-x