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- Title
Purification of a trypsin inhibitor from sweet potato in an aqueous two phase system.
- Authors
Jaw, Kuen-Shan; Chou, Lin Hung; Chang, Shiao-Ming; Duan, Kow-Jen
- Abstract
Trypsin inhibitor from sweet potato was extracted and purified in a single step using an aqueous two-phase system of polyethylene glycol 6000 (11% w/v), phosphate (16.5% w/v), KCl (9% w/v) and at pH 6. Purity of the trypsin inhibitor was enhanced 3.7-fold, and the recovery was 95%. The purified trypsin inhibitor showed one visible band, and the molecular size was 23 kDa by SDS-PAGE.
- Publication
Biotechnology letters, 2007, Vol 29, Issue 1, p137
- ISSN
1573-6776
- Publication type
Journal Article
- DOI
10.1007/s10529-006-9210-8