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- Title
Electron capture dissociation mass spectrometry in characterization of peptides and proteins.
- Authors
Bakhtiar, Ray; Guan, Ziqiang
- Abstract
Electron capture dissociation (ECD) represents one of the most recent and significant advancements in tandem mass spectrometry (MS/MS) for the identification and characterization of polypeptides. In comparison with the conventional fragmentation techniques, such as collisionally activated dissociation (CAD), ECD provides more extensive sequence fragments, while allowing the labile modifications to remain intact during backbone fragmentation--an important attribute for characterizing post-translational modifications. Herein, we present a brief overview of the ECD technique as well as selected applications in characterization of peptides and proteins. Case studies including characterization and localization of amino acid glycosylation, methionine oxidation, acylation, and "top-down" protein mass spectrometry using ECD will be presented. A recent technique, coined as electron transfer dissociation (ETD), will be also discussed briefly.
- Publication
Biotechnology letters, 2006, Vol 28, Issue 14, p1047
- ISSN
0141-5492
- Publication type
Journal Article
- DOI
10.1007/s10529-006-9065-z