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- Title
A genome-based approach to create a minimally mutated Corynebacterium glutamicum strain for efficient L-lysine production.
- Authors
Ikeda, Masato; Ohnishi, Junko; Hayashi, Mikiro; Mitsuhashi, Satoshi
- Abstract
Based on the progress in genomics, we have developed a novel approach that employs genomic information to generate an efficient amino acid producer. A comparative genomic analysis of an industrial L-lysine producer with its natural ancestor identified a variety of mutations in genes associated with L-lysine biosynthesis. Among these mutations, we identified two mutations in the relevant terminal pathways as key mutations for L-lysine production, and three mutations in central metabolism that resulted in increased titers. These five mutations when assembled in the wild-type genome led to a significant increase in both the rate of production and final L-lysine titer. Further investigations incorporated with transcriptome analysis suggested that other as yet unidentified mutations are necessary to support the L-lysine titers observed by the original production strain. Here we describe the essence of our approach for strain reconstruction, and also discuss mechanisms of L-lysine hyperproduction unraveled by combining genomics with classical strain improvement.
- Publication
Journal of industrial microbiology & biotechnology, 2006, Vol 33, Issue 7, p610
- ISSN
1367-5435
- Publication type
Journal Article
- DOI
10.1007/s10295-006-0104-5