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- Title
A microplate spectrofluorometric assay for bacterial biofilms.
- Authors
Burton, E; Yakandawala, N; LoVetri, K; Madhyastha, M S
- Abstract
A spectrofluorometric assay was developed for quantification of bacterial biofilms grown on a microtiter plate. The method involved staining biofilms formed by gram-negative and gram-positive bacteria with wheat germ agglutinin-Alexa Fluor 488 conjugate, which selectively binds to N-acetylglucosamine residues in biofilms. The fluorescence of stained biofilms was measured with a fluorescent plate reader. This method was compared with a widely used microplate colorimetric assay involving crystal violet staining of biofilms formed by both gram-negative and gram-positive bacteria. A strong linear association existed between the two methods (r (2)=0.99/0.94). Being more sensitive and specific as compared to colorimetric method, the spectrofluorometric assay provides a better alternative for quantification and characterization of bacterial biofilms.
- Publication
Journal of industrial microbiology & biotechnology, 2007, Vol 34, Issue 1, p1
- ISSN
1367-5435
- Publication type
Journal Article
- DOI
10.1007/s10295-006-0086-3