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- Title
Evaluation of the inactivation of infectious Herpes simplex virus by host-defense peptides.
- Authors
Yasin, B; Pang, M; Turner, J S; Cho, Y; Dinh, N N; Waring, A J; Lehrer, R I; Wagar, E A
- Abstract
A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide microplate assay was adapted to screen for the ability of 20 host-defense peptides to inactivate herpes simplex virus type 1 and type 2. The procedure required minimal amounts of material, was reproducible, and was confirmed with standard antiviral testing techniques. In screening tests, with the exception of melittin, a highly cytotoxic and hemolytic peptide found in bee venom, the alpha-helical peptides in our test panel (magainins, cecropins, clavanins, and LL-37) caused little viral inactivation. Several beta-sheet peptides (defensins, tachyplesin, and protegrins) inactivated one or both viruses, sometimes with remarkable selectivity. Two peptides were identified as having antiviral activity against both viruses, indolicidin (a tryptophan-rich peptide from bovine neutrophils) and brevinin-1 (a peptide found in frog skin). The antiviral activity of these two peptides was confirmed with standard antiviral assays. Interestingly, the antiviral activity of brevinin-1 was maintained after reduction and carboxamidomethylation, procedures that abolished its otherwise prominent hemolytic and cytotoxic effects.
- Publication
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2000, Vol 19, Issue 3, p187
- ISSN
0934-9723
- Publication type
Journal Article
- DOI
10.1007/s100960050457