Title

Porcine circovirus genotypes and their copathogens in pigs with respiratory disease in southern provinces of Vietnam.

Authors

Dinh, Phat Xuan; Nguyen, Minh Nam; Nguyen, Hien The; Tran, Vu Hoang; Tran, Quy Dinh; Dang, Kim Hoang; Vo, Dai Tan; Le, Hien Thanh; Nguyen, Nam Thi Thu; Nguyen, Toan Tat; Do, Duy Tien

Abstract

This study was conducted to investigate the genetic diversity of porcine circovirus type 2 (PCV2) and its coinfecting pathogens in pigs with respiratory disease in Vietnam. Samples from 127 clinical cases were obtained from different southern provinces of Vietnam from January 2018 to January 2020 for PCR and sequence analysis. The infection rate of PCV2 was 78.8%, and the major pathogens found in coinfections with PCV2 were porcine reproductive and respiratory syndrome virus, Mycoplasma hyopneumoniae, and Haemophilus parasuis. Forty-three PCV2-positive clinical samples were selected for amplification and sequencing of the ORF2 region. Phylogenetic analysis of PCV2 ORF2 showed that five of the sequences belonged to PCV2b (11.6%) and 38 belonged to PCV2d (88.4%), indicating that PCV2d strains were predominant in southern provinces of Vietnam. Alignment of the predicted amino acid sequences of the PCV2 capsid protein revealed polymorphic sites in the antibody recognition regions. This study demonstrates the prevalence of the PCV2d genotype in southern Vietnam and presents a comprehensive overview of the coinfecting pathogens associated with PCV2 in young pigs with respiratory disease.

Subjects

VIETNAM; CIRCOVIRUS diseases; PORCINE reproductive & respiratory syndrome; RESPIRATORY diseases; AMINO acid sequence; MYCOPLASMA hyopneumoniae; SWINE; GENOTYPES

Publication

Archives of Virology, 2021, Vol 166, Issue 2, p403

ISSN

0304-8608

Publication type

Academic Journal

DOI

10.1007/s00705-020-04878-y