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- Title
The transcription factor Gcr1 stimulates cell growth by participating in nutrient-responsive gene expression on a global level.
- Authors
Kellie Barbara; Terry Haley; Kristine Willis; George Santangelo
- Abstract
Abstract??Transcriptomic reprogramming is critical to the coordination between growth and cell cycle progression in response to changing extracellular conditions. InSaccharomyces cerevisiae, the transcription factor Gcr1 contributes to this coordination by supporting maximum expression of G1 cyclins in addition to regulating both glucose-induced and glucose-repressed genes. We report here the comprehensive genome-wide expression profiling ofgcr1? cells. Our data show that reduced expression of ribosomal protein genes ingcr1? cells is detectable both 20?min after glucose addition and in steady-state cultures of raffinose-grown cells, showing that this defect is not the result of slow growth or growth on a repressing sugar. However, the large cell phenotype of thegcr1? mutant occurs only in the presence of repressing sugars.GCR1deletion also results in aberrant derepression of numerous glucose repressed loci; glucose-growngcr1? cells actively respire, demonstrating that this global alteration in transcription corresponds to significant changes at the physiological level. These data offer an insight into the coordination of growth and cell division by providing an integrated view of the transcriptomic, phenotypic, and metabolic consequences ofGCR1deletion.
- Publication
Molecular Genetics & Genomics, 2007, Vol 277, Issue 2, p171
- ISSN
1617-4615
- Publication type
Academic Journal
- DOI
10.1007/s00438-006-0182-0