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- Title
Microarray and real-time PCR analyses reveal mating type-dependent gene expression in a homothallic fungus.
- Authors
Pöggeler, S; Nowrousian, M; Ringelberg, C; Loros, J J; Dunlap, J C; Kück, U
- Abstract
Sordaria macrospora is a homothallic ascomycete which is able to form fertile fruiting bodies without a mating partner. To analyze the molecular basis of homothallism and the role of mating products during fruiting body development, we have deleted the mating type gene Smta-1 encoding a high-mobility group domain (HMG) protein. The DeltaSmta-1 deletion strain is morphologically wild type during vegetative growth, but it is unable to produce perithecia or ascospores. To identify genes expressed under control of Smta-1, we performed a cross-species microarray analysis using Neurospora crassa cDNA microarrays hybridized with S. macrospora targets. We identified 107 genes that are more than twofold up- or down-regulated in the mutant. Functional classification revealed that 81 genes have homologues with known or putative functions. Comparison of array data from DeltaSmta-1 with those from three phenotypically similar mutants revealed that only a limited set of ten genes is deregulated in all mutants. Remarkably, the ppg2 gene encoding a putative lipopeptide pheromone is 500-fold down-regulated in the DeltaSmta-1 mutant while in all other sterile mutants this gene is up-regulated.
- Publication
Molecular genetics and genomics : MGG, 2006, Vol 275, Issue 5, p492
- ISSN
1617-4615
- Publication type
Journal Article
- DOI
10.1007/s00438-006-0107-y