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- Title
A survey of SL1-spliced transcripts from the root-lesion nematode Pratylenchus penetrans.
- Authors
Mitreva, M; Elling, A A; Dante, M; Kloek, A P; Kalyanaraman, A; Aluru, S; Clifton, S W; Bird, D McK; Baum, T J; McCarter, J P
- Abstract
Plant-parasitic nematodes are important and cosmopolitan pathogens of crops. Here, we describe the generation and analysis of 1928 expressed sequence tags (ESTs) of a splice-leader 1 (SL1) library from mixed life stages of the root-lesion nematode Pratylenchus penetrans. The ESTs were grouped into 420 clusters and classified by function using the Gene Ontology (GO) hierarchy and the Kyoto KEGG database. Approximately 80% of all translated clusters show homology to Caenorhabditis elegans proteins, and 37% of the C. elegans gene homologs had confirmed phenotypes as assessed by RNA interference tests. Use of an SL1-PCR approach, while ensuring the cloning of the 5' ends of mRNAs, has demonstrated bias toward short transcripts. Putative nematode-specific and Pratylenchus -specific genes were identified, and their implications for nematode control strategies are discussed.
- Publication
Molecular genetics and genomics : MGG, 2004, Vol 272, Issue 2, p138
- ISSN
1617-4615
- Publication type
Journal Article
- DOI
10.1007/s00438-004-1054-0