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- Title
Dominant-negative E-cadherin inhibits the invasiveness of inflammatory breast cancer cells in vitro.
- Authors
Dong, Hui-Ming; Liu, Gang; Hou, Yi-Feng; Wu, Jiong; Lu, Jin-Song; Luo, Jian-Min; Shen, Zhen-Zhou; Shao, Zhi-Ming
- Abstract
E-cadherin is a transmembrane glycoprotein which mediates epithelial cell-to-cell adhesion function as a tumor suppressor and frequently loss of expression in a wide spectrum of human cancer. However, recent studies demonstrated that E-cadherin was always over-expressed in inflammatory breast cancer (IBC) specimen and cell lines, which is a clinical extreme malignancy of breast cancer. It is hypothesized that the gain and not the loss of the E-cadherin axis contributes to the IBC unique phenotype. To test this assumption, we generated dominant negative mutant E-cadherin high-expression inflammatory breast cancer cells by introduced dominant negative mutant E-cadherin (H-2kd-E-cad) cDNA into human IBC SUM149 cells. Our studies demonstrated that the ability of invasion of SUM149 cells was significantly inhibited by H-2kd-E-cad via down-regulation of MMP-1 and MMP-9 expression. The underlying signal pathway of MAPK phosphorylated Erk 1/2(P44/42) in H-2kd-E-cad-transfected SUM149 cells was significantly down-regulated compared to parental and mock contrast. Our studies provided further functional evidence as the gain of E-cadherin expression dedicated to the IBC malignant phenotype and the blockage of MAPK/Erk activation maybe a promising therapeutic target.
- Publication
Journal of cancer research and clinical oncology, 2007, Vol 133, Issue 2, p83
- ISSN
0171-5216
- Publication type
Journal Article
- DOI
10.1007/s00432-006-0140-6