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- Title
Induction of differentiation and peroxisome proliferator-activated receptor ? expression in colon cancer cell lines by troglitazone.
- Authors
Masashi Kato; Tomomi Kusumi; Shigeki Tsuchida; Masanori Tanaka; Mutsuo Sasaki; Hajime Kudo
- Abstract
Purpose We investigated the relationship between the effects of troglitazone (TGZ) on cellular growth, differentiation and apoptosis induction, and the induction of peroxisome proliferator-activated receptor (PPAR) ? in three human colon cancer cell lines, HCT-15, DLD-1and LoVo. Methods Viable cell number was evaluated by the Alamar blue assay and apoptotic cell death by TUNEL methods. Expression of PPAR? mRNA and protein was examined by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot, respectively. The differentiation markers of colonic mucosa, villin and MUC2 mRNAs, were analyzed by real-time RT-PCR. Results HCT-15 and DLD-1 cells proliferated rapidly while LoVo cells grew slowly. TGZ dose-dependently inhibited the proliferation of all the cell lines, and also induced apoptotic cell death. High expression of PPAR? mRNA and protein was demonstrated in DLD-1 and LoVo cells before TGZ treatment. After the treatment, PPAR? mRNA and protein levels were increased in HCT-15 and LoVo cells. Villin and MUC2 mRNAs were increased by TGZ treatment in HCT-15 cells while villin mRNA was repressed in LoVo cells. Changes in expression of PPAR?, villin or MUC2 mRNAs were not observed in DLD-1 cells. Conclusions These results suggest that PPAR? levels are not correlated with the rates of cell proliferation. Differentiation induction by TGZ was only observed in the cell lines with enhanced PPAR? expression.
- Publication
Journal of Cancer Research & Clinical Oncology, 2004, Vol 130, Issue 2, p73
- ISSN
0171-5216
- Publication type
Academic Journal
- DOI
10.1007/s00432-003-0510-2