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- Title
Molecular analysis of theCRINKLY4gene family inArabidopsis thaliana.
- Authors
Xueyuan Cao; Kejian Li; Sang-Gon Suh; Tao Guo; Becraft, Philip W.
- Abstract
The maize (Zea maysL.)CRINKLY4(CR4) gene encodes a serine/threonine receptor-like kinase that controls an array of developmental processes in the plant and endosperm. TheArabidopsis thaliana(L.) Heynh. genome encodes an ortholog of CR4, ACR4, and four CRINKLY4-RELATED (CRR) proteins: AtCRR1, AtCRR2, AtCRR3 and AtCRK1. The available genome sequence of rice (Oryza sativaL.) encodes a CR4 ortholog, OsCR4, and four CRR proteins: OsCRR1, OsCRR2, OsCRR3 and OsCRR4, not necessarily orthologous to theArabidopsisCRRs. A phylogenetic study showed that AtCRR1 and AtCRR2 form a clade closest to the CR4 group while all the other CRRs form a separate cluster. The fiveArabidopsisgenes are differentially expressed in various tissues. A construct formed by fusion of theACR4promoter and theGUSreporter,ACR4::GUS, is expressed primarily in developing tissues of the shoot. The ACR4 cytoplasmic domain functions in vitro as a serine/threonine kinase, while the AtCRR1 and AtCRR2 kinases are not active. The ability of ACR4 to phosphorylate AtCRR2 suggests that they might function in the same signal transduction pathway. T-DNA insertions were obtained inACR4,AtCRR1,AtCRR2,AtCRR3andAtCRK1. Mutations inacr4show a phenotype restricted to the integuments and seed coat, suggesting thatArabidopsismight contain a redundant function that is lacking in maize. The lack of obvious mutant phenotypes in thecrrmutants indicates they are not required for the hypothetical redundant function.
- Publication
Planta: An International Journal of Plant Biology, 2005, Vol 220, Issue 5, p645
- ISSN
0032-0935
- Publication type
Academic Journal
- DOI
10.1007/s00425-004-1378-3