We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The dibenzodioxocin lignin substructure is abundant in the inner part of the secondary wall in Norway spruce and silver birch xylem.
- Authors
Eija M. Kukkola; Sanna Koutaniemi; Eija Pöllänen; Mikaela Gustafsson; Pirkko Karhunen; Taina K. Lundell; Pekka Saranpää; Ilkka Kilpeläinen; Teemu H. Teeri; Kurt V. Fagerstedt
- Abstract
A specific condensed lignin substructure, dibenzodioxocin, was immunolocalized in differentiating cell walls of Norway spruce ( Picea abies (L.) H. Karsten) and silver birch ( Betula pendula Roth) xylem. A fluorescent probe, Alexa 488 was used as a marker on the dibenzodioxocin-specific secondary antibody. For the detection of this lignin substructure, 25-µm cross-sections of xylem were viewed with a confocal laser-scanning microscope with fluorescein isothiocyanate fluorescence filters. In mature cells, fluorescence was detected in the S3 layer of the secondary wall in both tree species, but it was more intense in Norway spruce than in silver birch. In silver birch most of the signal was detected in vessel walls and less in fiber cell walls. In very young tracheids of Norway spruce and vessels and fibers of silver birch, where secondary cell wall layers were not yet formed, the presence of the dibenzodioxocin structure could not be shown.
- Publication
Planta: An International Journal of Plant Biology, 2004, Vol 218, Issue 3, p497
- ISSN
0032-0935
- Publication type
Academic Journal
- DOI
10.1007/s00425-003-1107-3