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- Title
Reversibility of exercise-induced translocation of Na<sup>+</sup>-K<sup>+</sup> pump subunits to the plasma membrane in rat skeletal muscle.
- Authors
Juel, Carsten; Grunnet, Louise; Holse, Mille; Kenworthy, Signe; Sommer, Viveca; Wulff, Tune
- Abstract
Exercise-induced translocation of Na+-K+ pump subunits to the sarcolemmal membrane was studied using sarcolemmal giant vesicles as a membrane purification procedure. The subunit content was quantified by Western blotting or by ouabain labeling. Low-intensity treadmill running increased (P<0.01) the α1, α2, β1, and β2 subunit contents by 19–32% in membranes from oxidative muscle fibers and the α1, α2, and β2 contents increased by 13–25% in membranes from glycolytic muscle fibers. Ouabain labeling of membranes from mixed fibers was increased by 29% after exercise. A similar increase in subunit content could be induced by 5 min of fatiguing, high-intensity electrical stimulation of isolated soleus muscles. An increased subunit content was just detectable in vesicles produced 30 min after exercise, and the content was completely back to control levels 3 h after exercise. It is concluded that both low-intensity long-lasting running and short-lasting high-intensity contractions are able to induce a translocation of pump subunits to the sarcolemmal membrane. The post-exercise disappearance of the extra subunits (half-time approximately 20 min) from the membrane demonstrates the reversible nature of the translocation process.
- Publication
Pflügers Archiv: European Journal of Physiology, 2001, Vol 443, Issue 2, p212
- ISSN
0031-6768
- Publication type
Academic Journal
- DOI
10.1007/s004240100674