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- Title
Next-generation sequencing of vertebrate experimental organisms.
- Authors
Turner, Daniel J; Keane, Thomas M; Sudbery, Ian; Adams, David J
- Abstract
Next-generation sequencing technologies are revolutionizing biology by allowing for genome-wide transcription factor binding-site profiling, transcriptome sequencing, and more recently, whole-genome resequencing. While it is currently not possible to generate complete de novo assemblies of higher-vertebrate genomes using next-generation sequencing, improvements in sequence read lengths and throughput, coupled with new assembly algorithms for large data sets, will soon make this a reality. These developments will in turn spawn a revolution in how genomic data are used to understand genetics and how model organisms are used for disease gene discovery. This review provides an overview of the current next-generation sequencing platforms and the newest computational tools for the analysis of next-generation sequencing data. We also describe how next-generation sequencing may be applied in the context of vertebrate model organism genetics.
- Publication
Mammalian genome : official journal of the International Mammalian Genome Society, 2009, Vol 20, Issue 6, p327
- ISSN
1432-1777
- Publication type
Journal Article
- DOI
10.1007/s00335-009-9187-4