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- Title
SNP2RFLP: a computational tool to facilitate genetic mapping using benchtop analysis of SNPs.
- Authors
Beckstead, Wesley A; Bjork, Bryan C; Stottmann, Rolf W; Sunyaev, Shamil; Beier, David R
- Abstract
Genome-wide analysis of single nucleotide polymorphism (SNP) markers is an extremely efficient means for genetic mapping of mutations or traits in mice. However, this approach often defines a relatively large recombinant interval. To facilitate the refinement of this interval, we developed the program SNP2RFLP. This program can be used to identify region-specific SNPs in which the polymorphic nucleotide creates a restriction fragment length polymorphism (RFLP) that can be readily assayed at the benchtop using restriction enzyme digestion of SNP-containing PCR products. The program permits user-defined queries that maximize the informative markers for a particular application. This facilitates fine-mapping in a region containing a mutation of interest, which should prove valuable to the mouse genetics community. SNP2RFLP and further details are publicly available at http://genetics.bwh.harvard.edu/snp2rflp/ .
- Publication
Mammalian genome : official journal of the International Mammalian Genome Society, 2008, Vol 19, Issue 10-12, p687
- ISSN
0938-8990
- Publication type
Journal Article
- DOI
10.1007/s00335-008-9149-2