We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A first-generation EST RH comparative map of the porcine and human genome.
- Authors
Rink, Anette; Santschi, Elizabeth M; Eyer, Katie M; Roelofs, Benjamin; Hess, Markus; Godfrey, Myra; Karajusuf, Elif K; Yerle, Martine; Milan, Denis; Beattie, Craig W
- Abstract
We have constructed a first-generation EST radiation hybrid comparative map of the porcine genome by assigning 1,058 markers to the IMpRH(7000) panel. Chromosomal localization was determined with a 2pt LOD of 4.8 for 984 markers, using the IMpRH mapping tool. Annotated ESTs represent 46.2% or 489 of the markers. Marker distribution was not stochastic and ranged from 0.41 for SSC8 to 1.77 for SSC12, respectively. Two hundred fifty-one markers assigned to the physical map of the pig did not find a homologous sequence in V22 of the human genome assembly, indicative of gaps in the assembled human genome sequence. The comparative porcine/human map covers 3,290 MB, or 98.3% of the presumed size of the human genome. However, 60 breakpoints were identified between chromosomes, as well as 90 micro-rearrangements within synteny groups. Six porcine chromosomes-SSC2, 5, 6, 7, 12, and 14-correspond to the three gene-richest human chromosomes, HSA17, 19, and 22, and show above average marker density. Porcine Chrs 1, 8, 11, and X display a low DNA/marker ratio and correspond to the 'genome deserts' on HSA 18, 4, 13, and X.
- Publication
Mammalian genome : official journal of the International Mammalian Genome Society, 2002, Vol 13, Issue 10, p578
- ISSN
0938-8990
- Publication type
Journal Article
- DOI
10.1007/s00335-002-2192-5