We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Analysis of a nonribosomal peptide synthetase gene from Alternaria brassicae and flanking genomic sequences.
- Authors
Guillemette, Thomas; Sellam, Adnane; Simoneau, Philippe
- Abstract
Very little information is currently available concerning the pathogenic determinants produced by Alternaria brassicae, the causal agent of the blackspot disease of crucifers. We screened a genomic library of this fungus and identified a nonribosomal peptide synthetase (NRPS) gene named AbrePsy1. The complete coding sequence is 22 kbp long and encodes a large protein (792 kDa) showing typical NRPS modular organization. Structural analysis of AbrePsy1 revealed four complete elongation modules, two of which have epimerization domains. In the vicinity of AbrePsy1, a second gene (named AbreAtr1), which encodes an ATP-binding cassette transporter was identified. Increased expression of AbrePsy1 and AbreAtr1 was observed during host-plant infection. However, while physically linked, these two genes are probably not functionally clustered, as their expression patterns differed.
- Publication
Current genetics, 2004, Vol 45, Issue 4, p214
- ISSN
0172-8083
- Publication type
Journal Article
- DOI
10.1007/s00294-003-0479-z