We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Early detection and monitoring of cartilage alteration in the experimental meniscectomised guinea pig model of osteoarthritis by <sup>99m</sup>Tc-NTP 15-5 scintigraphy.
- Authors
Miot-Noirault, Elisabeth; Vidal, Aurélien; Pastoureau, Philippe; Bonafous, Jacques; Chomel, Agnès; Sarry, Laurent; Audin, Laurent; Madelmont, Jean-Claude; Moins, Nicole
- Abstract
This study in the meniscectomised guinea pig aimed to demonstrate that the radiotracer 99mTc-NTP 15-5 would have pathophysiological validity for in vivo osteoarthritis imaging. The specificity of 99mTc-NTP 15-5 for cartilage was determined in healthy animals ( n = 13), by tissue radioactivity counting, joint autoradiography and scintigraphy. 99mTc-NTP 15-5 scintigraphy was performed at 20, 50, 80, 115, 130, 150 and 180 days after medial meniscectomy ( n = 10 MNX) or sham operation ( n = 5), and scintigraphic ratios (operated/contralateral) were calculated for femoral (F) and tibial (T) areas. F and T ratios were compared with those of 99mTc-MDP bone scintigraphy. At the study end-point, autoradiographic analysis of joint 99mTc-NTP 15-5 distribution and macroscopic scoring of cartilage integrity were performed. The high and specific accumulation of 99mTc-NTP 15-5 in normal cartilage (about 5.5 ± 1.7 % of injected dose/g of tissue), which permitted joint imaging with high contrast, was affected by osteoarthritis. In the MNX group, 99mTc-NTP 15-5 accumulation in cartilage within the operated joint, relative to the contralateral joint, was observed to change in the same animals as pathology progressed. Although F and T ratios were significantly higher in MNX (F = 1.7 ± 0.2; T = 1.6 ± 0.1) than in shams (F = 1.0 ± 0.1; T = 1.0 ± 0.1) at day 50, they were significantly lower in MNX (F = 0.6 ± 0.1; T = 0.7 ± 0.1) than in shams (F = 1.0 ± 0.1; T = 0.9 ± 0.1) at day 180. No change in 99mTc-MDP uptake was observed over 6 months. Macroscopic analysis confirmed features of osteoarthritis only in MNX knees. These results in MNX guinea pigs provide additional support for the use of 99mTc-NTP 15-5 for in vivo imaging of osteoarthritis.
- Publication
European Journal of Nuclear Medicine & Molecular Imaging, 2007, Vol 34, Issue 8, p1280
- ISSN
1619-7070
- Publication type
Academic Journal
- DOI
10.1007/s00259-006-0320-2